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minimal residual disease MRD, which is mutated in patients with the complication. The 2,569 available samples were obtained from 346 patients with AML who had undergone prior intensive treatment. Additionally, a panel of 51 personalized genes was used to perform specific sequencing of 223 samples obtained at diagnosis and 49 samples collected at the time of relapse. Finally, mutations associated with preleukemic clones were screened by digital PCR. Most of the transplants used are based on the direct application to the site of the injury or close to it of a small amount of cells in suspension through multiple injections. Other forms of administration that have been tested are the introduction of these same cells through the intrathecal or systemic route, with results, however, very questionable.
With few exceptions, in the spinal cord injury model in small animals, such as experimental mice, they have been transplanted in the acute phases, during the first or second week after the injury, and there are few studies in chronic models, due to to the difficulty of keeping animals alive after causing serious spinal cord injury. Unlike what happens in humans, where the studies carried out in phases I and II are trials in chronic patients with more than a year of evolution and, therefore, the possible benefits of these techniques are more difficult to observe.
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